Loss of Deoxyribonucleic Acid-Thymine During Thymine Starvation of Escherichia coli

Loss of deoxyribonucleic acid-thymine during thymine starvation of Escherichia coli.

A substantial loss of deoxyribonucleic acid-thymine occurs during thymine starvation of several thymine auxotrophs derived from Escherichia coli strains B, 15, and K-12.

Influence of thymine starvation on the integrity of deoxyribonucleic acid in Escherichia coli.

The influence of thymine starvation on the single-strand molecular weight of deoxyribonucleic acid (DNA) from Escherichia coli was determined by sedimentation through gradients of alkaline sucrose. Growth of cells for as long as 150 min in thymineless medium did not significantly reduce the molecular weight below the control value of 2.4 +/- 0.3 x 10(8) daltons. Incubation of cells in thyminele...

Thymine Starvation and Single-Strand Breaks in Chromosomal Deoxyribonucleic acid of Escherichia coli.

Single-strand breaks, as measured by the McGrath and Williams procedure, occur in chromosomal deoxyribonucleic acid of Escherichia coli cells during thymine starvation.

Alkaline sucrose gradient sedimentation of chromosomal deoxyribonucleic acid from Escherichia coli PolA + and PolA - strains during thymine starvation.

Single-strand breaks were not detected in the deoxyribonucleic acid of Escherichia coli after thymine starvation for up to 180 min, even in a sensitive PolA(-) strain.

Technique for starvation of Escherichia coli of thymine.